منابع مشابه
Hydroxyl radical footprints reveal novel structural features around the NF I binding site in adenovirus DNA.
We have identified a number of as yet unknown structural abnormalities of the NF I-DNA binding site within the inverted terminal repetition of adenovirus DNA by probing it with a hydroxyl radical footprinting technique. NF I binding alters the accessibility of the deoxyribose moieties to hydroxyl radicals both at the 3' and at the 5' side of the recognition sequence 5'-TGG(N)6GCCAA-3'. A smooth...
متن کاملHydroxyl radical mediated DNA base modification by manmade mineral fibres.
Manmade mineral fibres (MMMFs) were examined for their ability to hydroxylate 2-deoxyguanosine (dG) to 8-hydroxydeoxyguanosine (8-OH-dG), a reaction that is mediated by hydroxyl radicals. It appeared that (1) catalase and the hydroxyl radical scavengers, dimethylsulphoxide and sodium benzoate, inhibited the hydroxylation, whereas Fe2+ and H2O2 potentiated it; (2) pretreatment of MMMFs with the ...
متن کاملFree-radical-mediated DNA binding.
Free-radical metabolites can be generated metabolically by a one-electron reductase-catalyzed reaction or a "peroxidase" catalyzed oxidation or by photoactivation of a wide variety of aromatic xenobiotics. Radicals may also be generated during lipid peroxidation. Some radicals can react with DNA or bind covalently or noncovalently as a dismutation product or as a dimer, trimer or polymeric prod...
متن کاملNew rhodamine nitroxide based fluorescent probes for intracellular hydroxyl radical identification in living cells.
The synthesis, characteristics, and biological applications of a series of new rhodamine nitroxide fluorescent probes that enable imaging of hydroxyl radicals (•OH) in living cells are described. These probes are highly selective for •OH in aqueous solution, avoiding interference from other reactive oxygen species (ROS), and they facilitate •OH imaging in biologically active samples. The robust...
متن کاملDesigning photoluminescent molecular probes for singlet oxygen, hydroxyl radical, and iron–oxygen species
Metabolism of molecular oxygen in the human body inevitably produces reactive oxygen species (ROS). Among the family of ROS, those exhibiting very high reactivities, including _OH, _OOH, ONOO , HOCl, HOBr, and O2, remain relatively underexplored. While emerging evidence suggests that these ROS are critically involved in physiological function as well as the genesis and development of diseases, ...
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ژورنال
عنوان ژورنال: Radiation Physics and Chemistry
سال: 2012
ISSN: 0969-806X
DOI: 10.1016/j.radphyschem.2011.09.006